OPTIMIZED CENTRIFUGATION FOR RAPID ELUTION OF DNA FROM AGAROSE GELS

被引：16

作者：

HE, MY

LIU, H

WANG, YX

AUSTEN, B

机构：

[1] ST GEORGE HOSP,SCH MED,DEPT SURG,CRANMER TERRACE,LONDON SW17 0RE,ENGLAND

[2] UNIV LONDON UNIV COLL,MRC,HUMAN BIOCHEM GENET UNIT,LONDON WC1E 6BT,ENGLAND

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1992年 / 9卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/1050-3862(92)90027-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method has been developed for the isolation of DNA from agarose gels. Centrifugation in a low-cost device for 45 s at low speed provides a high yield of DNA suitable for further manipulation by restriction enzymes, T4 DNA ligase, Taq polymerase, Klenow fragment, and T4 polynucleotide kinase, and also for sequencing. In contrast, centrifugation for >1 min leads to coelution of substances that inhibit DNA ligase.

引用

页码：31 / 33

页数：3