EVIDENCE LINKING THE 68-KILODALTON ANTIGEN IDENTIFIED IN PROGRESSIVE SENSORINEURAL HEARING-LOSS PATIENT SERA WITH HEAT-SHOCK PROTEIN 70

被引:118
作者
BILLINGS, PB
KEITHLEY, EM
HARRIS, JP
机构
[1] UNIV CALIF SAN DIEGO, DIV OTOLARYNGOL HEAD & NECK SURG, LA JOLLA, CA 92093 USA
[2] VET ADM MED CTR, SAN DIEGO, CA USA
关键词
AUTOIMMUNE INNER EAR DISEASE; AUTOIMMUNITY; SENSORINEURAL HEARING LOSS; STRESS PROTEINS;
D O I
10.1177/000348949510400302
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
Immunoblotting against bovine inner ear extracts has previously identified a 68 kd antigen reactive with 22% to 58% of sera of patients with rapidly progressive sensorineural hearing loss (PSNHL) of suspected autoimmune causation. Efforts to purify and characterize this diagnostic antigen suggest that it is ubiquitous rather than inner ear-specific, and may represent the highly inducible heat shock protein (hsp) 70. The antigens identified by PSNHL sera and anti-hsp 70 monoclonal antibodies copurify on ion exchange and adenosine triphosphate affinity chromatography, and comigrate on one- and two-dimensional gel electrophoresis. Additionally, immunoblotting with positive patient sera shows dramatically increased expression of the 68 kd antigen by bovine kidney cells following heat shock in culture. Reactivity with stress proteins of various classes has been reported in a number of autoimmune diseases; however, anti-hsp 70 appears uniquely associated with ulcerative colitis and PSNHL.
引用
收藏
页码:181 / 188
页数:8
相关论文
共 22 条
[1]   EVIDENCE OF SERUM ANTIBODIES AGAINST INNER-EAR TISSUES IN THE BLOOD OF PATIENTS WITH CERTAIN SENSORINEURAL HEARING DISORDERS [J].
ARNOLD, W ;
PFALTZ, R ;
ALTERMATT, HJ .
ACTA OTO-LARYNGOLOGICA, 1985, 99 (3-4) :437-444
[2]  
BROOKES GB, 1986, ARCH OTOLARYNGOL, V112, P536
[3]   THE CONSTITUTIVE AND STRESS INDUCIBLE FORMS OF HSP-70 EXHIBIT FUNCTIONAL SIMILARITIES AND INTERACT WITH ONE ANOTHER IN AN ATP-DEPENDENT FASHION [J].
BROWN, CR ;
MARTIN, RL ;
HANSEN, WJ ;
BECKMANN, RP ;
WELCH, WJ .
JOURNAL OF CELL BIOLOGY, 1993, 120 (05) :1101-1112
[4]   FOLDING OF NASCENT POLYPEPTIDE-CHAINS IN A HIGH-MOLECULAR-MASS ASSEMBLY WITH MOLECULAR CHAPERONES [J].
FRYDMAN, J ;
NIMMESGERN, E ;
OHTSUKA, K ;
HARTL, FU .
NATURE, 1994, 370 (6485) :111-117
[5]  
HARRIS JP, 1990, LARYNGOSCOPE, V100, P516
[6]   MOLECULAR CHAPERONES IN PROTEIN-FOLDING - THE ART OF AVOIDING STICKY SITUATIONS [J].
HARTL, FU ;
HLODAN, R ;
LANGER, T .
TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (01) :20-25
[7]  
HUGHES GB, 1994, AM J OTOL, V15, P198
[8]  
HUGHES GB, 1983, LARYNGOSCOPE, V93, P410
[9]   AUTOANTIBODIES TO HUMAN STRESS PROTEINS - A SURVEY OF VARIOUS RHEUMATIC AND OTHER INFLAMMATORY DISEASES [J].
JARJOUR, WN ;
JEFFRIES, BD ;
DAVIS, JS ;
WELCH, WJ ;
MIMURA, T ;
WINFIELD, JB .
ARTHRITIS AND RHEUMATISM, 1991, 34 (09) :1133-1138
[10]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+