RBL2P, A YEAST PROTEIN THAT BINDS TO BETA-TUBULIN AND PARTICIPATES IN MICROTUBULE FUNCTION IN-VIVO

被引：76

作者：

ARCHER, JE ^{[1
]}

VEGA, LR ^{[1
]}

SOLOMON, F ^{[1
]}

机构：

[1] MIT,CTR CANC RES,CAMBRIDGE,MA 02139

来源：

CELL | 1995年 / 82卷 / 03期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0092-8674(95)90431-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic configurations resulting in high ratios of beta-tubulin to alpha-tubulin are toxic in S. cerevisiae, causing microtubule disassembly and cell death, We identified three non-tubulin yeast genes that, when overexpressed, rescue cells from excess beta-tubulin, One, RBL2, rescues beta-tubulin lethality as efficiently as does alpha-tubulin. Rbl2p binds to beta-tubulin in vivo, Deficiencies or excesses of either Rbl2p or alpha-tubulin affect microtubule-dependent functions in a parallel fashion, Rbl2p has functional homology with murine cofactor A, a protein important for in vitro assays of beta-tubulin folding. The results suggest that Rbl2p participates in microtubule morphogenesis but not in the assembled polymer.

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页码：425 / 434

页数：10

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