USE OF FLUORESCEIN CONJUGATED ANTISERA TO IDENTIFY PROTEINS ON STAINED AND DRIED ELECTROPHORESIS STRIPS

被引：2

作者：

FENTON, JCB

SHINE, BSF

机构：

[1] Department of Chemical Pathology, St. Bartholomew's Hospital, London, EC1A 7BE, West Smithfield

来源：

CLINICA CHIMICA ACTA | 1979年 / 92卷 / 02期

关键词：

D O I：

10.1016/0009-8981(79)90125-6

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Paraprotein bands discovered on routine electrophoresis may be identified immunologically without repeating the electrophoretic separation. It is only necessary to heat the stained and dried strip in a hot air oven to reactivate the antigenic sites and insolubilise the protein. The strip may then be stained using specific antisera conjugated with fluorescein. The absence of prozone phenomena greatly helps in typing of free light chains. © 1979.

引用

页码：289 / 292

页数：4