INHIBITION OF DEACYLATION AND IMPROVEMENT IN N-HYDROXYSUCCINIMIDE ESTER MODIFICATION OF PHENYLALANYL-TRANSFERRNA BY DIMETHYL-SULFOXIDE

被引：11

作者：

REUBEN, MA ^{[1
]}

KUSNEZOV, IJ ^{[1
]}

WICKSTROM, E ^{[1
]}

机构：

[1] UNIV DENVER,DEPT CHEM,DENVER,CO 80208

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 565卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

Aminoacyl-tRNA; N-Hydroxysuccinimide ester modification; tRNA deacylation; tRNA modification;

D O I：

10.1016/0005-2787(79)90099-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have found that dimethyl sulfoxide significantly inhibits deacylation of Phe-tRNA. This allows a high pH in N-hydroxysuccinimide ester reactions while maintaining a high level of aminoacylated tRNA, improving the overall yield of the Phe-tRNA modification reaction. © 1979.

引用

页码：219 / 223

页数：5

共 13 条

[1] A GENERAL PROCEDURE FOR ISOLATION OF SPECIFIC TRANSFER RIBONUCLEIC ACIDS [J].