INDUCTION OF SPECIFIC ANTI-ACTIN ANTIBODIES AND THEIR MEASUREMENT BY ELISA TECHNIQUE

被引:14
作者
CHARLEMAGNE, D
SCHWARTZ, K
BERNARD, S
PARAF, A
机构
[1] INRA,RECH VIROL & IMMUNOL STN,F-78850 THIVERVAL GRIGNON,FRANCE
[2] HOP LARIBOISIERE,INSERM,U127,UNITE RECH METAB COEUR & VAISSEAUX,F-75475 PARIS 10,FRANCE
关键词
D O I
10.1016/0022-1759(79)90064-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Using the ELISA technique we have been able to quantify antibodies directed against actin and to follow the kinetics of antibody production. Specific anti-actin antisera have been raised in rabbits by immunization with chemically modified white muscle rabbit actin. Two or three dinitrophenyl groups linked per actin molecule were sufficient to break natural tolerance, while linkage of three phosphorylcholine groups to actin was not. © 1979.
引用
收藏
页码:145 / 153
页数:9
相关论文
共 18 条
[1]   CROSS-LINKING OF PROTEINS WITH GLUTARALDEHYDE AND ITS USE FOR PREPARATION OF IMMUNOADSORBENTS [J].
AVRAMEAS, S ;
TERNYNCK, T .
IMMUNOCHEMISTRY, 1969, 6 (01) :53-+
[2]   ANTI-ACTIN [J].
BRAY, D .
NATURE, 1974, 251 (5472) :187-187
[3]   ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) QUANTITATIVE ASSAY OF IMMUNOGLOBULIN-G [J].
ENGVALL, E ;
PERLMANN, P .
IMMUNOCHEMISTRY, 1971, 8 (09) :871-&
[4]   THE PREPARATION AND SOME PROPERTIES OF PURIFIED ANTIBODY SPECIFIC FOR THE 2,4-DINITROPHENYL GROUP [J].
FARAH, FS ;
KERN, M ;
EISEN, HN .
JOURNAL OF EXPERIMENTAL MEDICINE, 1960, 112 (06) :1195-1210
[5]   PRODUCTION OF SPECIFIC ANTIBODIES TO CONTRACTILE PROTEINS, AND THEIR USE IN IMMUNOFLUORESCENCE MICROSCOPY .2. SPECIES-SPECIFIC AND SPECIES-NON-SPECIFIC ANTIBODIES TO SMOOTH AND STRIATED CHICKEN MUSCLE ACTIN [J].
GROSCHELSTEWART, U ;
CEURREMANS, S ;
LEHR, I ;
MAHLMEISTER, C ;
PAAR, E .
HISTOCHEMISTRY, 1977, 50 (04) :271-279
[6]  
JOCKUSH BM, 1978, HISTOCHEMISTRY, V55, P1
[7]   ANTIBODIES TO TUBULIN IN NORMAL NONIMMUNIZED ANIMALS - (NATURAL ANTIBODIES-PEROXIDASE-LABELED ANTIBODIES-AUTO-ANTIBODIES-IMMUNOADSORBENTS) [J].
KARSENTI, E ;
GUILBERT, B ;
BORNENS, M ;
AVRAMEAS, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (09) :3997-4001
[8]   G-F EQUILIBRIUM IN ACTIN SOLUTIONS UNDER VARIOS CONDITIONS [J].
KASAI, M ;
OOSAWA, F ;
ASAKURA, S .
BIOCHIMICA ET BIOPHYSICA ACTA, 1962, 57 (01) :13-&
[9]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[10]   ACTIN ANTIBODY - SPECIFIC VISUALIZATION OF ACTIN-FILAMENTS IN NON-MUSCLE CELLS [J].
LAZARIDES, E ;
WEBER, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (06) :2268-2272