SELECTIVE PROTEIN SEPARATIONS USING FORMED-IN-PLACE ANION-EXCHANGE MEMBRANES

被引：9

作者：

LI, YH ^{[1
]}

SPENCER, HG ^{[1
]}

机构：

[1] CLEMSON UNIV,DEPT CHEM,CLEMSON,SC 29634

来源：

JOURNAL OF BIOTECHNOLOGY | 1992年 / 26卷 / 2-3期

关键词：

SELECTIVE PROTEIN SEPARATION; ION EXCHANGE MEMBRANE; SEQUENTIAL CYCLIC SEPARATION PROCEDURE;

D O I：

10.1016/0168-1656(92)90007-V

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Anion exchange membranes prepared by adsorption of polymers on Formed-In-Place microfiltration substrates were formed and ion-exchange separations of solutions containing two proteins were determined by ion exchange membrane sequential separation procedures, similar to affinity membrane separation procedures. Representative ion exchange separation processes utilizing adsorbed poly(ethylene imine) (PEI) as the ion exchange membrane for the separation of the components of solutions containing two proteins, bovine serum albumin (BSA) and lysozyme and ovalbumin and lysozyme, are described. The stability of the PEI adsorbed layer, binding characteristics of the BSA to the membrane and purification properties of the procedure were determined.

引用

页码：203 / 211

页数：9

共 9 条

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