BINDING OF EXOGENOUS DNA BY CHROMATIN FROM ISOLATED PLANT-CELL NUCLEI

被引：4

作者：

BLASCHEK, W ^{[1
]}

机构：

[1] LEHRSTUHL BOT ENTWICKLUNGSPHYSIOL,INST GENET 02400,D-7000 STUTTGART 70,FED REP GER

来源：

PLANT SCIENCE LETTERS | 1979年 / 15卷 / 02期

关键词：

D O I：

10.1016/0304-4211(79)90066-X

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

DNA-binding and uptake by nuclei isolated from protoplasts of Petunia hybrida were investigated using radioactive DNA prepared from E. coli. Isolated nuclei were incubated with bacterial DNA. After washing the nuclei the nuclear sap was extracted with 0.14 M NaCl/buffer. Chromatin was released from the nuclei by sonication and banded by isopycnic centrifugation in metrizamide gradients. Detection of chromatin was achieved by fluorescence measurement with ethidium bromide. In the nuclear sap bacterial DNA was found and an association between chromatin and bacterial DNA was demonstrated. A covalent integration of the bacterial DNA into the plant DNA could not be detected. © 1979.

引用

页码：139 / 149

页数：11

共 27 条

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