DETERMINATION OF CYSTEINE ON LOW-DENSITY LIPOPROTEINS USING THE FLUORESCENT-PROBE, 5-IODOACETAMIDOFLUORESCEINE

被引：37

作者：

COLEMAN, RD ^{[1
]}

KIM, TW ^{[1
]}

GOTTO, AM ^{[1
]}

YANG, CY ^{[1
]}

机构：

[1] BAYLOR UNIV,METHODIST HOSP,DEPT MED,6565 FANNIN,MS A-601,HOUSTON,TX 77030

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1037卷 / 01期

关键词：

Amino acid sequence; Apolipoprotein B-100; Fluorescent labeling; Lipoprotein (a); Low density lipoproteins; Sulfhydryl peptide;

D O I：

10.1016/0167-4838(90)90111-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Using the fluorescent sulfhydryl probe, 5-iodoacetamidofluoresceine, to label the free sulfhydryl of low-density lipoprotein, the positions of two cysteine residues in apolipoprotein B were located. The tryptic peptides containing the fluorescent probe were isolated by high-performance liquid chromatography systems and sequenced by automatic techniques. The free cysteine residues of apolipoprotein B-100 on low-density lipoprotein are located at positions 3734 and 4190, either or both of which can potentially form a disulfide linkage with apolipoprotein(a) in lipoprotein(a). © 1990.

引用

页码：129 / 132

页数：4

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