EFFICIENT EXPRESSION AND ZN(II)-DEPENDENT STRUCTURE OF THE DNA-BINDING DOMAIN OF THE YEAST GAL4 PROTEIN

被引：6

作者：

SERIKAWA, Y ^{[1
]}

SHIRAKAWA, M ^{[1
]}

MATSUO, H ^{[1
]}

KYOGOKU, Y ^{[1
]}

机构：

[1] OSAKA UNIV,INST PROT RES,SUITA,OSAKA 565,JAPAN

来源：

PROTEIN ENGINEERING | 1990年 / 3卷 / 04期

关键词：

DNA binding domain; GAL4; NMR; Zn-dependent conformation; Zn-finger;

D O I：

10.1093/protein/3.4.267

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three protein fragments of different sizes which contain the DNA binding domain of transcription factor GAL4 from Saccharomyces cerevisiae have been expressed in functional forms in Escherichia coli. DNase I footprinting and gel retardation assays showed that the purified proteins bound to the same DNA sequence on the gal1-gal10 promoter as intact GAL4 does. Denaturation-refolding experiments demonstrated that Zn(II) is necessary for maintenance of the conformation of the DNA binding domain of GAL4, as judged on UV-CD and 1H-NMR measurements, as well as for specific DNA binding. © 1990 Oxford University Press.

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页码：267 / 272

页数：6

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