RECONSTITUTION OF THE CORE COMPLEX (ALPHA-BETA)(3)(APC)L(C)(8.9) OF THE PHYCOBILISOME FROM MASTIGOCLADUS-LAMINOSUS USING THE L(C)(8.9) LINKER POLYPEPTIDE OVEREXPRESSED IN ESCHERICHIA-COLI

被引：11

作者：

BETZ, M ^{[1
]}

RUEGSEGGER, U ^{[1
]}

ESTEBAN, AM ^{[1
]}

SIDLER, WA ^{[1
]}

ZUBER, H ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,HPM,INST MOLEK BIOL & BIOPHYS,CH-8093 ZURICH,SWITZERLAND

来源：

BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1993年 / 374卷 / 07期

关键词：

PROTEIN STRUCTURE; PHOTOSYNTHESIS; CYANOBACTERIA; PHYCOBILIPROTEIN; ALLOPHYCOCYANIN; APCC GENE;

D O I：

10.1515/bchm3.1993.374.7-12.435

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The apcC gene from Mastigocladus laminosus encodes the linker polypeptide L(C)8.9 located in the phycobilisome core. AT7 RNA polymerase expression system was used to express the linker polypeptide L(C)8.9 from M. laminosus in Escherichia coli. The apcC gene product was expressed as an inclusion body which was solubilized in a buffer containing 8m urea. Final purification was achieved by ion exchange chromatography on Fractogel TSK CM 650 (S). In addition, a method for preparative isolation of the L(C)8.9 linker polypeptide from M. laminosus by reverse phase chromatography is presented. Both L(C)8.9 isolated from M. laminosus and overexpressed in E. coli were capable of reconstituting the complex (alphabeta)3(APC)L(C)8.9. The reconstituted complex was identical to preparations isolated from M. laminosus in terms of polypeptide composition, absorption and fluorescence emission spectroscopy.

引用

页码：435 / 443

页数：9

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