SPIN TRANSITION OF CAMPHOR-BOUND CYTOCHROME-P-450 .1. LOCAL PAH AND ELECTROSTATIC INTERACTIONS

被引：24

作者：

LANGE, R

DEBEY, P

机构：

[1] Institut de Biologie Physico-Chimique, Paris, F-75005

[2] Unité 128 de L'institut National de la Santé Et de la Recherche Médicale, Montpellier, F-34033

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 94卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb12916.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Variations of the spin state in camphor‐bound cytochrome P‐450 are interpreted in the light of the polyelectrolyte theory and its implications on the microenvironment of the heme. The ratio of high‐spin to low‐spin iron can serve as a tool to determine the local paH in the microenvironment of a group (pK0,app∼ 5.4) which governs the spin state. The local paH depends on the electrostatic potential created by negatively charged groups (pKa= 5.6), modulated in turn by paH and by the screening effect of ionic strength. A model is given for the proton‐coupled spin state change. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：485 / 489

页数：5

共 16 条

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[2]

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