BIOTECHNOLOGICAL PRODUCTION OF D-AMINO OR L-AMINO-ACIDS FROM 5-MONOSUBSTITUTED HYDANTOINS

被引：14

作者：

SYLDATK, C

WAGNER, F

机构：

[1] Institute of Biochemistry and Biotechnology, Technical University of Braunschweig, Braunschweig

来源：

FOOD BIOTECHNOLOGY | 1990年 / 4卷 / 01期

关键词：

D O I：

10.1080/08905439009549725

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The great advantage of producing optically pure D-or L-amino acids from D, L-5-monosubstituted hydantoins by bioconversion is the fact that most of the substrates already racemize under reaction conditions and are totally converted to the optically pure amino acids. Three enzymes were shown to be involved in the production of L-tryptophan from D, L-5-indolylmethylhydantoin in Arthrobacter spec. DSM 3747 after growth in a medium containing the chemically synthesized D.L-5-indolyl-3-N-methlylhydantoin as effective inducer. Besides a hydantoinase and a N-carbamoyl-L-amino acid amidohydrolase, a 5-indolylmethylhydantoin racemase was found and could be separated from the former enzymes by liquid-liquid extraction and gel filtration. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：87 / 95

页数：9

共 17 条

[1]

Battilotti M., Barberini U., Preparation of D-valine from D,L-5-isopropylhydantoin by stereoselective biocatalysis, J. Mol. Cat., 43, pp. 343-352, (1988)

[2]

Bradford M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding, Anal. Biochem., 72, pp. 248-254, (1976)

[3]

Dombach G., Isolierung und Charakterisierung einer Hydantoinase aus, Arthrobacter spec., (1989)

[4]

Gross C., Syldatk C., Wagner F., Development of a process for the production of aromatic L-amino acids from D, L-5-monosubstituted hydantoins, Proc. 4th Europ. Congress Biotechnol., 2, pp. 249-252, (1987)

[5]

Guivarch M., Gillonier C., Brunie J.-C., Obtention d’amino acides obtiquement actifs a 1’ aide d’hydantoinases, Bull. Soc. Chim. Fr., 1-2, pp. 91-95, (1980)

[6]

Nishida Y., Nakamichi K., Nabe K., Tosa T., Enzymatic production of L-tryptophan from D, L-5-indolylmethylhydantoin, Flavobacterium spec., 9, pp. 721-725, (1987)

[7]

Moller A., Syldatk C., Schulze M., Wagner F., Stereo-and substrate specificity of a D-hydantoinase and a D-N-carbamylamino acid amidohydrolase, Arthrobacter crystallopoietes AM2. Enzyme Microb. Technol., 10, pp. 618-625, (1988)

[8]

Muller R., Syldatk C., Wagner F., Isolation and characterization of a N-carbamoyl-L-amino acid amidohydrolase, Arthrobacter spec., 3, (1989)

[9]

Schmidt-Kastner G., Egerer P., Amino acids and peptides, Biotechnology, 6 a, pp. 387-419, (1984)

[10]

Soda K., Tanaka H., Esaki N., Amino acids, Biotechnology, 3, pp. 480-530, (1983)

← 1 2 →