DROSOPHILA CDC2 HOMOLOGS - A FUNCTIONAL HOMOLOG IS COEXPRESSED WITH A COGNATE VARIANT

被引:176
作者
LEHNER, CF [1 ]
OFARRELL, PH [1 ]
机构
[1] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143
关键词
cdc2; cdc2-cognate; cell cycle; complementation; Drosophila;
D O I
10.1002/j.1460-2075.1990.tb07568.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using probes obtained by PCR amplification, we have cloned Drosophila cDNAs encoding structural homologs of the p34(cdc2) cell cycle kinase. Southern blot experiments and in situ hybridization to polytene chromosomes demonstrated that the isolated cDNAs, were derived from two distinct genes, Dm cdc2 (31E) and Dm cdc2c (92F). Northern blot and in situ hybridization experiments revealed that these two genes are coexpressed during embryogenesis and that expression is correlated with cell proliferation. However, despite the similarity in structure and expression, the two gene products differed in functional assays in yeasts. Expression of Dm cdc2 in Schizosaccharomyces pombe and Saccharomyces cerevisiae rescued cell cylce arrest caused by mutations in cdc2+ and CDC28, the genes encoding the p34(cdc2) kinase homologs of these yeasts. In contrast, the Dm cdc2c gene product did not restore cell cycle progression. Thus, in addition to the identification of a functional homolog in Drosophila, our results indicate the presence of a closely related cognate of the p34(cdc2) cell cycle kinase.
引用
收藏
页码:3573 / 3581
页数:9
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