CHEMILUMINESCENT ASSAY FOR THE DETECTION OF VIRAL AND PLASMID DNA USING DIGOXIGENIN-LABELED PROBES

被引：45

作者：

MUSIANI, M ^{[1
]}

ZERBINI, M ^{[1
]}

GIBELLINI, D ^{[1
]}

GENTILOMI, G ^{[1
]}

LAPLACA, M ^{[1
]}

FERRI, E ^{[1
]}

GIROTTI, S ^{[1
]}

机构：

[1] UNIV BOLOGNA,IST SCI CHIM,I-40138 BOLOGNA,ITALY

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 194卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90247-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A dot-blot hybridization immunoenzymatic assay with a chemiluminescent endpoint was developed for the rapid and sensitive detection of viral and plasmid DNAs. Digoxigenin-labeled probes were used to detect cytomegalovirus, parvovirus B19, and plasmid pBR328 DNAs. Hybridized probes were immunoenzy-matically visualized by anti-digoxigenin Fab fragments labeled with alkaline phosphatase, and adamantyl 1,2-dioxetane phenyl phosphate was used as chemiluminescent substrate. Results were recorded by instant photographic films. The chemiluminescent hybridization assay was performed in about 8 hr and was able to detect as little as 50-10 fg of homologous target DNA. © 1991.

引用

页码：394 / 398

页数：5

共 23 条

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