BACULOVIRUS-EXPRESSED HERPES-SIMPLEX VIRUS TYPE-2 GLYCOPROTEIN-D IS IMMUNOGENIC AND PROTECTIVE AGAINST LETHAL HSV CHALLENGE

被引:24
作者
LANDOLFI, V [1 ]
ZARLEY, CD [1 ]
ABRAMOVITZ, AS [1 ]
FIGUEROA, N [1 ]
WU, SL [1 ]
BLASIAK, M [1 ]
ISHIZAKA, ST [1 ]
MISHKIN, EM [1 ]
机构
[1] LEDERLE PRAXIS BIOL, DEPT VIRAL VACCINE RES & DEV, PEARL RIVER, NY 10965 USA
关键词
HERPES SIMPLEX VIRUS; GLYCOPROTEIN-D; BACULOVIRUS EXPRESSION; IMMUNOGENICITY; PROTECTIVITY;
D O I
10.1016/0264-410X(93)90280-B
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Herpes simplex virus type 2 glycoprotein D (gD2) was cloned and expressed in the baculovirus-Spodoptera frugiperda system. Milligram quantities of glycoprotein were recovered from suspension culture and subjected to purification by ion-exchange and immunoaffinity chromatography. The resultant purified gD existed as a homogeneous 57 500 M(W) monomeric species demonstrating reactivity with anti-gD monoclonal antibodies including those directed at a non-sequential neutralizing epitope of gD. Immunization of Balb/c mice with doses of 0.1-10.0 mug of AlPO4-absorbed gD resulted in elicitation of humoral and cellular responses to both HSV1 and HSV2 as well as to purified gD1 and gD2. Immunized mice receiving an infectious dose of 1 x 10(6) p.f.u. of HSV2 via the footpad route were significantly protected against infection at all doses tested when compared with unimmunized AlPO4 and uninoculated control animals.
引用
收藏
页码:407 / 414
页数:8
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