BACULOVIRUS-EXPRESSED HERPES-SIMPLEX VIRUS TYPE-2 GLYCOPROTEIN-D IS IMMUNOGENIC AND PROTECTIVE AGAINST LETHAL HSV CHALLENGE

被引：24

作者：

LANDOLFI, V ^{[1
]}

ZARLEY, CD ^{[1
]}

ABRAMOVITZ, AS ^{[1
]}

FIGUEROA, N ^{[1
]}

WU, SL ^{[1
]}

BLASIAK, M ^{[1
]}

ISHIZAKA, ST ^{[1
]}

MISHKIN, EM ^{[1
]}

机构：

[1] LEDERLE PRAXIS BIOL, DEPT VIRAL VACCINE RES & DEV, PEARL RIVER, NY 10965 USA

来源：

VACCINE | 1993年 / 11卷 / 04期

关键词：

HERPES SIMPLEX VIRUS; GLYCOPROTEIN-D; BACULOVIRUS EXPRESSION; IMMUNOGENICITY; PROTECTIVITY;

D O I：

10.1016/0264-410X(93)90280-B

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Herpes simplex virus type 2 glycoprotein D (gD2) was cloned and expressed in the baculovirus-Spodoptera frugiperda system. Milligram quantities of glycoprotein were recovered from suspension culture and subjected to purification by ion-exchange and immunoaffinity chromatography. The resultant purified gD existed as a homogeneous 57 500 M(W) monomeric species demonstrating reactivity with anti-gD monoclonal antibodies including those directed at a non-sequential neutralizing epitope of gD. Immunization of Balb/c mice with doses of 0.1-10.0 mug of AlPO4-absorbed gD resulted in elicitation of humoral and cellular responses to both HSV1 and HSV2 as well as to purified gD1 and gD2. Immunized mice receiving an infectious dose of 1 x 10(6) p.f.u. of HSV2 via the footpad route were significantly protected against infection at all doses tested when compared with unimmunized AlPO4 and uninoculated control animals.

引用

页码：407 / 414

页数：8

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