EFFECT OF ACETONITRILE IN THE SAMPLING SOLUTION ON THE ANALYTE PEAK SHAPE IN MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

被引：15

作者：

CRABTREE, HJ ^{[1
]}

IRELAND, ID ^{[1
]}

DOVICHI, NJ ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2G2,AB,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 669卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)80358-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

When acetone-2,4-dinitrophenylhydrazone is dissolved in pure acetonitrile and analyzed by micellar electrokinetic capillary chromatography, peak splitting is observed. As the amount of acetonitrile is reduced in the sample solution, the peaks converge and a single, narrow peak is observed. Peak splitting due to geometric isomerism has previously been observed in gas and liquid chromatographic analyses of dinitrophenylhydrazine derivatives of aldehydes and asymmetrical ketones, but the acetone derivative has no such isomerism. Peak splitting is thought to be a result of the interaction of acetonitrile with the micelles in the separation buffer. This artifact may occur whenever analytes are prepared in a buffer containing a large concentration of organic phase; decreasing the concentration of organic phase should produce simpler electropherograms.

引用

页码：263 / 267

页数：5

共 11 条

[1] EFFECT OF PH ON THE REACTION OF 2,4-DINITROPHENYLHYDRAZINE WITH FORMALDEHYDE AND ACETALDEHYDE [J].