ANTIOXIDANT ENZYMATIC SYSTEMS AND OXIDATIVE STRESS IN ERYTHROCYTES WITH G6PD DEFICIENCY - EFFECT OF DEFEROXAMINE

被引：9

作者：

VANELLA, A ^{[1
]}

CAMPISI, A ^{[1
]}

CASTORINA, C ^{[1
]}

SORRENTI, V ^{[1
]}

ATTAGUILE, G ^{[1
]}

SAMPERI, P ^{[1
]}

AZZIA, N ^{[1
]}

DIGIACOMO, C ^{[1
]}

SCHILIRO, G ^{[1
]}

机构：

[1] UNIV CATANIA,INST PEDIAT HAEMATOL,I-95124 CATANIA,ITALY

来源：

PHARMACOLOGICAL RESEARCH | 1991年 / 24卷 / 01期

关键词：

HRBC; FAVISM; DEFEROXAMINE;

D O I：

10.1016/1043-6618(91)90061-2

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In the present study we have assayed the effect of divicine in G6PD-deficient red blood cells in the presence of deferoxamine (iron-chelating drug) and NaN3 (inhibitor of catalase). The effect of divicine has been compared to oxidative stress by H2O2; haemolysis is regarded as an index of cellular toxicity. In addition, we have tested antioxidant enzymatic systems. No significant change in antioxidant enzymatic systems was found in RBCs from subjects with G6PD deficiency when compared to the control group, either in oxidative haemolysis by divicine or by H2O2; a significant decrease in oxidative haemolysis by H2O2 was observed in the presence of deferoxamine, whereas no change was found in oxidative haemolysis by divicine. The replacement of incubation medium by homologous plasma or the supplementation with bovine serum albumin resulted in a marked decrease of percentage of haemolysis by divicine. © 1991 The Italian Pharmacological Society.

引用

页码：25 / 31

页数：7

共 23 条

[1] DETECTION OF A FREE-RADICAL INTERMEDIATE FROM DIVICINE OF VICIA-FABA [J].

ALBANO, E ;

TOMASI, A ;

MANNUZZU, L ;

ARESE, P .

BIOCHEMICAL PHARMACOLOGY, 1984, 33 (10) :1701-1704

[2]

ARESE P, 1986, GLUCOSE 6 PHOSPHATE, P45

[3]

BAKER MA, 1983, THESIS COLUMBIA U NE

[4]

BERGMEYER HU, 1965, METHOD ENZYMAT AN, P744

[5] THE HEMOLYTIC EFFECT OF PRIMAQUINE AND RELATED COMPOUNDS - A REVIEW [J].

BEUTLER, E .

BLOOD, 1959, 14 (02) :103-139

[6]

BEUTLER E, 1983, METABOLIC BASIS INHE, P1

[7]

CAMPISI A, 1988, Medical Science Research, V16, P693

[8] THE CHEMISTRY OF FAVISM-INDUCING COMPOUNDS - THE PROPERTIES OF ISOURAMIL AND DIVICINE AND THEIR REACTION WITH GLUTATHIONE [J].

CHEVION, M ;

NAVOK, T ;

GLASER, G ;

MAGER, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1982, 127 (02) :405-409

[9]

Chiu D, 1982, FREE RADICAL BIO MED, V5, P115

[10] ACTIVITY OF DIVICINE IN PLASMODIUM-VINCKEI-INFECTED MICE HAS IMPLICATIONS FOR TREATMENT OF FAVISM AND EPIDEMIOLOGY OF G-6-PD DEFICIENCY [J].

CLARK, IA ;

COWDEN, WB ;

HUNT, NH ;

MAXWELL, LE ;

MACKIE, EJ .

BRITISH JOURNAL OF HAEMATOLOGY, 1984, 57 (03) :479-487

← 1 2 3 →