BIOCONVERSION OF DETHIOBIOTIN INTO BIOTIN BY RESTING CELLS AND PROTOPLASTS OF BACILLUS-SPHAERICUS BIOB TRANSFORMANT

被引:7
作者
FUJISAWA, A [1 ]
ABE, T [1 ]
OHSAWA, I [1 ]
SHIOZAKI, S [1 ]
KAMOGAWA, K [1 ]
IZUMI, Y [1 ]
机构
[1] TOTTORI UNIV, FAC ENGN, DEPT BIOTECHNOL, TOTTORI 680, JAPAN
关键词
D O I
10.1271/bbb.57.740
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The reaction system for the bioconversion of dethiobiotin into biotin by resting cells and protoplasts of a Bacillus sphaericus bioB transformant was established. The reaction mixtures consisted of completely synthetic components, such as amino acids and metal salts. Among the sulfur compounds tested, L-Cys and L-cystine were effective in the biosynthesis of biotin from dethiobiotin both by resting cells and by protoplasts. The optimum concentrations of L-Cys were 2 to 3 mM and more than 0.25 mM for resting cell and protoplast systems, respectively. Vigorous shaking enhanced the biotin biosynthesis by protoplasts. The addition of yeast extract to the reaction mixture without a mixture of amino acids brought about a three-fold increase in the amount of biotin synthesized by protoplasts when compard to the case with the reaction mixture containing the amino acid mixture. The amount of biotin synthesized by protoplasts increased with the incubation time up to 6 h and reached about 2 mug/ml. There was a clear correlation between the number of remaining protoplasts and their biotin-biosynthesizing activity during the incubation.
引用
收藏
页码:740 / 744
页数:5
相关论文
共 16 条
  • [1] EISENBERG MA, 1973, ADV ENZYMOL RAMB, V38, P317
  • [2] CLONING AND CHARACTERIZATION OF THE BACILLUS-SPHAERICUS GENES-CONTROLLING THE BIOCONVERSION OF PIMELATE INTO DETHIOBIOTIN
    GLOECKLER, R
    OHSAWA, I
    SPECK, D
    LEDOUX, C
    BERNARD, S
    ZINSIUS, M
    VILLEVAL, D
    KISOU, T
    KAMOGAWA, K
    LEMOINE, Y
    [J]. GENE, 1990, 87 (01) : 63 - 70
  • [3] CONVERSION OF DETHIOBIOTIN TO BIOTIN IN CELL-FREE-EXTRACTS OF ESCHERICHIA-COLI
    IFUKU, O
    KISHIMOTO, J
    HAZE, S
    YANAGI, M
    FUKUSHIMA, S
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (11) : 1780 - 1785
  • [4] CHARACTERIZATION OF BIOTIN BIOSYNTHETIC-ENZYMES OF BACILLUS-SPHAERICUS - A DETHIOBIOTIN PRODUCING BACTERIUM
    IZUMI, Y
    KANO, Y
    INAGAKI, K
    KAWASE, N
    TANI, Y
    YAMADA, H
    [J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1981, 45 (09): : 1983 - 1989
  • [5] INCORPORATION OF SULFUR OF L-[S-35S]METHIONINE INTO BIOTIN MOLECULE BY INTACT CELLS OF RHODOTORULA-GLUTINIS
    IZUMI, Y
    SUGISAKI, K
    TANI, Y
    OGATA, K
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 304 (03) : 887 - 890
  • [6] IZUMI Y, 1980, B I CHEM RES KYOTO U, V58, P434
  • [7] LEMOINE Y, 1992, J FERMENT BIOENG, V73, P121
  • [8] STUDIES ON FORMATION OF BIOTIN FROM DESTHIOBIOTIN + SULFATE IN SACCHAROMYCES CEREVISIAE .3. ISOTOPIC STUDIES ON BIOTIN FORMATION FROM DESTHIOBIOTIN + S35-LABELLED SULFATE BY WASHED CELL SUSPENSION OF YEAST
    NIIMURA, T
    SAHASHI, Y
    SUZUKI, T
    [J]. JOURNAL OF VITAMINOLOGY, 1964, 10 (03): : 231 - +
  • [9] NIIMURA T, 1967, VITAMINS, V35, P444
  • [10] CLONING OF THE BIOTIN SYNTHETASE GENE FROM BACILLUS-SPHAERICUS AND EXPRESSION IN ESCHERICHIA-COLI AND BACILLI
    OHSAWA, I
    SPECK, D
    KISOU, T
    HAYAKAWA, K
    ZINSIUS, M
    GLOECKLER, R
    LEMOINE, Y
    KAMOGAWA, K
    [J]. GENE, 1989, 80 (01) : 39 - 48