PLASMINOGEN-ACTIVATOR INHIBITOR-1 MESSENGER-RNA IS EXPRESSED IN PLATELETS AND MEGAKARYOCYTES AND THE MEGAKARYOBLASTIC CELL-LINE CHRF-288

被引：45

作者：

KONKLE, BA ^{[1
]}

SCHICK, PK ^{[1
]}

HE, XL ^{[1
]}

LIU, RJ ^{[1
]}

MAZUR, EM ^{[1
]}

机构：

[1] ST MARYS HOSP, WATERBURY, CT USA

来源：

ARTERIOSCLEROSIS AND THROMBOSIS | 1993年 / 13卷 / 05期

关键词：

PLASMINOGEN ACTIVATOR INHIBITOR-1; PLATELETS; MEGAKARYOCYTES; FIBRINOLYSIS;

D O I：

10.1161/01.ATV.13.5.669

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Plasminogen activator inhibitor-1 (PAI-1) is present in the platelet alpha-granule and is released on platelet activation. Platelet PAI-1 could either be synthesized by the megakaryocyte or taken up from the plasma. In this report we confirm the presence of PAI-1 protein in human megakaryocytes by Western blot analysis and show its synthesis in guinea pig megakaryocytes by metabolic labeling. We document the presence of PAI-1 mRNA in human platelets and show a 3-kb mRNA species on Northern blot analysis of guinea pig megakaryocytes. Neither untreated CHRF-288 cells, a megakaryoyblastic cell line, nor human erythroleukemia (HEL) cells expressed PAI-1 mRNA. Phorbol ester (phorbol 12-myristate 13-acetate, 160 nM) treatment of CHRF-288 and HEL cells for 4 days induced PAI-1 mRNA expression in CHR F-288 cells but not in HEL cells. These studies show that PAI-1 is synthesized by megakaryocytes. Megakaryocytes most likely determine the PAI-1 content of platelets and thereby establish the antifibrinolytic potential of the platelet.

引用

页码：669 / 674

页数：6

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[1] VITRONECTIN BINDS TO ACTIVATED HUMAN PLATELETS AND PLAYS A ROLE IN PLATELET-AGGREGATION [J].