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THE PP60(C-SRC) IN RETINAL-PIGMENT EPITHELIUM AND ITS MODULATION BY VASOACTIVE-INTESTINAL-PEPTIDE

被引：9
作者：
KOH, SWM
机构：
[1] Department of Ophthalmology, University of Maryland Room 5-00C, 10 S. Pine Street, Baltimore
来源：
CELL BIOLOGY INTERNATIONAL REPORTS | 1992年 / 16卷 / 10期
关键词：
D O I：
10.1016/S0309-1651(06)80053-6
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The pp60c-src is present at high level in differentiated retinal pigment epithelium (RPE) in culture. Immunofluorescence microscopy using GD11 (anti-pp60c-src) shows intense staining in the plasma membrane, especially at the cell-cell junctions, and diffuse staining in the cytoplasma. Western blot analysis shows that the majority of the GD11-reactive molecules is localized in the membrane. The pp60c-src does not translocate between membrane and cytoplasma when the RPE was reacted with vasoactive intestinal peptide (VIP), which is previously shown to stimulate phosphorylation of the pp60c-src in the membranes (Biochem. Biophys. Res. Commun. 174, 452-8, 1991). Here, VIP modulation is shown to alter the reactivity of pp60c-src with a monoclonal anti-phosphotyrosine antibody. © 1992 Academic Press Ltd.
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页码：1003 / 1014
页数：12
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