MONITORING OF BENZODIAZEPINES (CLOBAZAM, DIAZEPAM AND THEIR MAIN ACTIVE METABOLITES) IN HUMAN PLASMA BY COLUMN-SWITCHING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：25

作者：

LACROIX, C

WOJCIECHOWSKI, F

DANGER, P

机构：

[1] Laboratoire de Pharmacocinétique, Centre Hospitalier

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 617卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80500-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A column-switching high-performance liquid chromatographic method for the simultaneous determination of clobazam, diazepam and their main metabolites in human plasma is described. A 200-mul plasma sample was directly injected into a precolumn filled with TSK-gel PW. After a washing step with potassium phosphate buffer, the retained substances were backflushed into a reversed-phase column with a mobile phase of acetonitrile-phosphate buffer-diethylamine. Various drugs frequently co-administered with clobazam or diazepam do not interfere with the determination.

引用

页码：285 / 290

页数：6

共 29 条

[1] DETERMINATION OF 11 BENZODIAZEPINE DERIVATIVES IN ACETONE SOLUTION BY GAS-CHROMATOGRAPHY UTILIZING NITROGEN AS A SPECIFIC DETECTOR [J].