ALDOSTERONE INHIBITS NITRIC-OXIDE SYNTHESIS IN RAT VASCULAR SMOOTH-MUSCLE CELLS INDUCED BY INTERLEUKIN-1-BETA

被引：77

作者：

IKEDA, U ^{[1
]}

KANBE, T ^{[1
]}

NAKAYAMA, I ^{[1
]}

KAWAHARA, Y ^{[1
]}

YOKOYAMA, M ^{[1
]}

SHIMADA, K ^{[1
]}

机构：

[1] KOBE UNIV,SCH MED,DEPT MED,DIV 1,KOBE 650,JAPAN

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1995年 / 290卷 / 02期

关键词：

INTERLEUKIN-1; NITRITE; NITRIC OXIDE (NO) SYNTHASE;

D O I：

10.1016/0922-4106(95)90018-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We investigated the effects of aldosterone on nitric oxide (NO) synthesis in vascular smooth muscle cells. We measured the production of nitrite, a stable metabolite of NO, and the expression of inducible NO synthase mRNA and protein in cultured rat vascular smooth muscle cells. incubation of the cultures with interleukin-1 beta (10 ng/ml) for 24 h caused a significant increase in nitrite generation. The interleukin-1 beta-induced nitrite production by vascular smooth muscle cells was significantly inhibited by aldosterone in a dose (10(-9) similar to 10(-6) M)-dependent manner. Incubation with interleukin-1 beta for 12 similar to 24 h caused inducible NO synthase mRNA expression in vascular smooth muscle cells, whereas aldosterone had a suppressive effect on its expression. Aldosterone also decreased interleukin-1 beta-induced inducible NO synthase protein accumulation. These results indicate that aldosterone inhibits NO synthesis under interleukin-1 beta-stimulated conditions in vascular smooth muscle cells.

引用

页码：69 / 73

页数：5

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