IDENTIFICATION OF A 13-KDA PROTEIN ASSOCIATED WITH THE POLYHYDROXYALKANOIC ACID GRANULES FROM ACINETOBACTER SPP

被引：23

作者：

SCHEMBRI, MA ^{[1
]}

WOODS, AA ^{[1
]}

BAYLY, RC ^{[1
]}

DAVIES, JK ^{[1
]}

机构：

[1] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 133卷 / 03期

关键词：

ACINETOBACTER; POLYHYDROXYALKANOIC ACID (PHA); POLY-BETA-HYDROXYBUTYRATE (PHB); PHA GRANULES; PHASINS; ACTIVATED SLUDGE;

D O I：

10.1016/0378-1097(95)00374-E

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Proteins associated with poly-beta-hydroxybutyrate (PHB) granules were purified from four Acinetobacter strains isolated from modified activated sludge treatment plants. Four predominant proteins of 63 kDa, 41 kDa, 38 kDa and 13 kDa were identified. N-terminal amino acid sequencing of the 64-kDa and 13-kDa proteins from Acinetobacter RA3849 identified these proteins as the products of the phaC(Ac) and phaP(Ac) (formerly designated ORF1) genes, respectively. The expression of the 13-kDa protein (referred to as GA13) is shown to be required for the accumulation of large amounts of PHB in a recombinant Escherichia coli strain.

引用

页码：277 / 283

页数：7

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