IDENTIFICATION AND GENETIC CONTROL OF 2 RABBIT ALPHA2-MACROGLOBULIN ALLOTYPES

被引:27
作者
KNIGHT, KL
DRAY, S
机构
[1] Department of Microbiology, University of Illinois, Medical Center, Chicago
关键词
D O I
10.1021/bi00843a038
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rabbits were immunized with macroglobulin preparations isolated from the serum of other rabbits by ultracentrifugation and gel filtration. This elicited precipitating isoantibodies which distinguished two antigenically different genetic variants, i.e., allotypes of serum α2-macroglobulin (α2M). Identification of these allotypes as α2M was based on the specific precipitation of a highly purified protein preparation which migrated electrophoretically as an α2-globulin and had a sedimentation coefficient of approximately 18 S. That the inheritance of these two allotypes is controlled by a pair of allelic genes is based on the allotypes present in 235 progeny from the six possible mating combinations; e.g., when both parents were heterozygous, the expected 1:2:1 distribution of progeny was observed (P = 0.95-0.9). This α2M locus, designated Mtz, was shown not to be linked to the X chromo-some nor to the light-chain or heavy-chain loci of the immunoglobulins. The use of these allotypes for the study of biosynthesis and structure of α2M is discussed. © 1968, American Chemical Society. All rights reserved.
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页码:1165 / &
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