DEHYDROGENASE-SILICA AS A STATIONARY PHASE FOR THE SEPARATION OF ALCOHOLS AND KETONES

被引：1

作者：

BIRNBAUM, S

NILSSON, KGI

机构：

[1] UNIV LUND,CTR CHEM,DEPT BIOTECHNOL,BOX 124,S-22100 LUND,SWEDEN

[2] UNIV LUND,CTR CHEM,DEPT PURE & APPL BIOCHEM,S-22100 LUND,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 587卷 / 02期

关键词：

D O I：

10.1016/0021-9673(91)85164-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Horse liver alcohol dehydrogenase was immobilized on tresyl-activated silica particles and slurry packed into a PTFE-coated stainless-steel column. The stationary phase enzyme column was used for the high-performance chromatographic separation of alcohols and ketones. Baseline resolution of the secondary alcohols terbutaline and bambuterol and their respective ketones was obtained. In addition, discrimination of closely related isomers, including the positional isomers of ethylphenethyl alcohol and the optical isomers of 1-phenyl-1-butanol, was achieved.

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页码：268 / 270

页数：3

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