ISOZYME COMPARISONS FOR IDENTIFICATION OF COLLETOTRICHUM SPECIES PATHOGENIC TO STRAWBERRY

被引:48
作者
BONDE, MR [1 ]
PETERSON, GL [1 ]
MAAS, JL [1 ]
机构
[1] USDA ARS, BELTSVILLE AGR RES CTR, FRUIT LAB, BELTSVILLE, MD 20705 USA
关键词
D O I
10.1094/Phyto-81-1523
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Presently there is uncertainty about the appropriate taxonomic classification of species of Colletotrichum infecting strawberry. Some workers consider C. fragariae part of the highly variable species C. gloeosporioides, whereas others feel they should be separate. The strawberry pathogens C. acutatum, C. fragariae, and C. gloeosporioides were successfully distinguished by means of comparing isozymes for 12 enzymes and 14 putative isozyme loci. The intraspecific coefficients of similarity (CS) based on the 12 enyzmes and 14 loci were 1.00 for C. acutatum (maximum possible = 1.00), 1.00 for C. fragariae, and 0.80 for C. gloeosporioides. Intraspecific CS values indicated little variation in C. acutatum and C. fragariae; however there was considerable variation in C. gloeosporioides. The interspecific CS comparing C. fragariae and C. gloeosporioides was relatively low (0.42), suggesting C. fragariae and C. gloeosporioides are distinct species. The considerably higher CS of 0.77 when comparing C. acutatum and C gloeosporioides suggests that they are more closely related. C. coccodes (= C. atramentarium) and C. trifolii also were included in the comparison. Neither exhibited intraspecific isozyme variation and further demonstrated the great potential for isozyme analysis to identify Colletotrichum species.
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页码:1523 / 1528
页数:6
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