FLUOROMETRIC ASSAY OF D-LACTATE

被引：89

作者：

MCLELLAN, AC

PHILLIPS, SA

THORNALLEY, PJ

机构：

[1] Department of Chemistry and Biological Chemistry, University of Essex, Colchester, C04 3SQ, Wivenhoe Park

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 206卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/S0003-2697(05)80004-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A fluorimetric assay for n-lactate in human blood samples was developed using an endpoint enzymatic assay with d-lactate dehydrogenase from Staphylococcus epidermidis. The intrabatch and interbatch coefficients of variance were 8.7% (n = 4) and 16.6% (n = 4), respectively. The limit of detection in blood was 3.73 nmol/ml. The assay suffers minor interference from S-d-lactoylglutathione, which was also present in the blood samples. The concentration of n-lactate in blood was (mean ± SE, nmol/ml) normal healthy individuals, 11.0 ± 1.2 (n = 7); and diabetic patients, 20.0 ± 1.3 (n = 55) (a significant increase in diabetes mellitus; P < 0.01, Mann-Whitney U test). © 1992 Academic Press, Inc.

引用

页码：12 / 16

页数：5

共 24 条

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[3]

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