MODULATION OF FATTY-ACID-BINDING PROTEIN-CONTENT OF ADULT-RAT HEART IN RESPONSE TO CHRONIC CHANGES IN PLASMA-LIPID LEVELS

被引：16

作者：

GARNIER, A ^{[1
]}

POIZAT, C ^{[1
]}

KERIEL, C ^{[1
]}

CUCHET, P ^{[1
]}

VORK, MM ^{[1
]}

DEJONG, YF ^{[1
]}

GLATZ, JFC ^{[1
]}

机构：

[1] UNIV LIMBURG,CARDIOVASC RES INST MAASTRICHT,DEPT PHYSIOL,6200 MD MAASTRICHT,NETHERLANDS

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1993年 / 123卷 / 1-2期

关键词：

FATTY ACID-BINDING PROTEIN; LIPID METABOLISM; CLOFIBRATE; INTRALIPID; CHRONIC HYPOXIA; RAT HEART;

D O I：

10.1007/BF01076481

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The aim of this work was to study in the adult rat heart the effect of modifications of fatty acid (FA) supply on the content of cytoplasmic fatty acid-binding protein (H-FABP(c)). To modify the amount of circulating lipids, three different treatments were chosen: (i) an hypolipidemic treatment with Clofibrate, administered daily through a gastric tube at a dose of 250 mg/kg per day for one week, (ii) a continuous intravenous infusion of 20% Intralipid, a fat emulsion, for one week at a dose of 96 ml/kg per day, and (iii) a normobaric hypoxia exposure (PO2 = 10%) for three weeks. At the end of each treatment plasma lipids, myocardial H-FABP(c) content and the activities of three key enzymes (citrate synthase, CS, fructrose-6-phosphate kinase, FPK and hydroxy-acyl CoA-dehydrogenase, HAD) were assessed. With each of the three treatments a decrease of plasma cholesterol and phospholipid levels was observed. Plasma FA concentration increased with Intralipid infusion and decreased with chronic hypoxia. The heart H-FABP(c) content was increased by 20% with Clofibrate, decreased by 20% with chronic hypoxia and remained unaltered upon Intralipid treatment. The induced changes in H-FABP(c) content were not related directly to changes in plasma lipid levels. CS activity was slightly decreased in the hypoxia group, FPK activity decreased in the Clofibrate group, and HAD activity decreased in the Intralipid group. Among the various groups heart H-FABP(c) content was related to HAD activity. In conclusion, the H-FABP(c) content of adult rat heart appears responsive to changes in plasma lipid levels.

引用

页码：107 / 112

页数：6

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