INCREASED SENSITIVITY OF DIAGNOSTIC LATEX AGGLUTINATION TESTS IN AN ULTRASONIC STANDING-WAVE FIELD

被引：28

作者：

GRUNDY, MA ^{[1
]}

MOORE, K ^{[1
]}

COAKLEY, WT ^{[1
]}

机构：

[1] UNIV WALES COLL CARDIFF, SCH PURE & APPL BIOL, CARDIFF CF1 3TL, S GLAM, WALES

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 176卷 / 02期

关键词：

HIGH SENSITIVITY AGGLUTINATION TEST; LATEX AGGLUTINATION TEST; ULTRASONIC STANDING WAVE FIELD; TUBULAR TRANSDUCER; ESCHERICHIA COLI O157; C-REACTIVE PROTEIN;

D O I：

10.1016/0022-1759(94)90311-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A technique is described which increases the sensitivity of latex agglutination tests for soluble and particulate antigens. The levels of detection of tests for C-reactive protein and E. coli O157 respectively have been improved by x 256 and x 1024 compared with the standard test procedure of sample rotation on a test-card. This new method combines dilution of the test latex particles, a 2 min sample treatment in the ultrasonic standing wave field of a tubular piezo-electric transducer and subsequent examination by video-microscopy. Ultrasonic treatment is required to achieve increased localised concentrations of the latex particles in the standing wave field, and dilution of the latex is a critical requirement to allow agglutination to occur at low antigen concentrations.

引用

页码：169 / 177

页数：9

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