DEPENDENCE OF ELECTROOSMOTIC FLOW IN CAPILLARY ELECTROPHORESIS ON GROUP-I AND GROUP-II METAL-IONS

被引：26

作者：

DICKENS, JE ^{[1
]}

GORSE, J ^{[1
]}

EVERHART, JA ^{[1
]}

RYAN, M ^{[1
]}

机构：

[1] BALDWIN WALLACE COLL,DEPT CHEM,BEREA,OH 44017

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 657卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00074-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of Group I and II metal ions on electroosmotic flow in capillary electrophoresis in fused-silica capillaries is characterized. The electroosmotic mobility of aqueous mobile phases of lithium, sodium, potassium, calcium and barium acetates in fused-silica capillaries is measured as a function of pH at constant voltage. Cross contamination is avoided by using separate columns for each study and pH control is maintained with the aid of He sparging. The shape of a plot of pH vs. electroosmotic mobility depends on the particular cation used which in turn depends on the surface sorption properties of the ions. Column history is demonstrated to have an effect on electroosmotic flow and therefore retention times. The resolution of a test mixture is optimal in the lithium-based buffer.

引用

页码：401 / 407

页数：7

共 18 条

[1]

ALTRIA KD, 1986, ANAL P, V23, P453

[2]

[Anonymous], 1979, CHEM SILICA

[3] FACTORS THAT INFLUENCE MOBILITY, RESOLUTION AND SELECTIVITY IN CAPILLARY ZONE ELECTROPHORESIS .2. THE ROLE OF THE BUFFERS CATION [J].