SIMPLE AND SENSITIVE METHOD FOR THE PURIFICATION AND PEPTIDE-MAPPING OF PROTEINS SOLUBILIZED FROM DENSONUCLEOSIS VIRUS WITH SODIUM DODECYL-SULFATE

An efficient method for the proteolysis and subsequent analysis of dansylated viral (or other) proteins solubilized with sodium dodecyl sulfate (SDS), after their purification using SDS electrophoresis, is described. The dansylation of proteins or the by-products of the reaction do not interfere in this technique. This very simple technique has important advantages over other methods for the purification and characterization of proteins. The method used indicates that the four viral proteins of densonucleosis virus originate at least partially from a common DNA sequence. © 1979.