CHANGES IN NUCLEAR AND POLYSOMAL POLYADENYLATED RNA SEQUENCES DURING RAT-LIVER REGENERATION

被引:32
作者
WILKES, PR
BIRNIE, GD
PAUL, J
机构
[1] Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Bearsden, Glasgow, G61, Garscube Estate, Switchback Road
关键词
D O I
10.1093/nar/6.6.2193
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nuclear and polysomal polyadenylated RNA popalations of normal and 16 hour regenerating rat liver have been compared by mRNA-cDNA hybridisations and by unique DNA saturation experiments. It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenerating rat liver. However, cross-hybridisation experiments using cDNA have shown that 10-15% by weight of nuclear polyadenylated RNA sequences are specific to 16 hour regenerating rat-liver. Since both unique DNA and cINA hybridisation have shown that normal and 16 hour regenerating rat-liver polysomal polyadenylated RNA populations are qualitatively very similar sequences specific to 16 hour regenerating rat-liver nuclear polyadenylated RNA are nucleus confined. Polysanal RNA sequences which were abundant in normal rat-liver have become less abundant in regenerating rat liver. © 1979 Information Retrieval Limited.
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页码:2193 / 2208
页数:16
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