IMAGING CYTOMETRY BY MULTIPARAMETER FLUORESCENCE

被引：50

作者：

GALBRAITH, W ^{[1
]}

WAGNER, MCE ^{[1
]}

CHAO, J ^{[1
]}

ABAZA, M ^{[1
]}

ERNST, LA ^{[1
]}

NEDERLOF, MA ^{[1
]}

HARTSOCK, RJ ^{[1
]}

TAYLOR, DL ^{[1
]}

WAGGONER, AS ^{[1
]}

机构：

[1] CARNEGIE MELLON UNIV, CTR FLUORESCENCE RES BIOMED SCI, PITTSBURGH, PA 15213 USA

来源：

CYTOMETRY | 1991年 / 12卷 / 07期

关键词：

IMAGE PROCESSING; QUANTITATION; LYMPHOCYTES;

D O I：

10.1002/cyto.990120702

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A system is described for performing multicolor fluorescence image cytometry of cell preparations. After the setting up stage, the operation is automatic: the microscope fields are found and focused; then images are acquired for each fluorophore, corrected and analyzed, without any operator interaction. Human peripheral blood lymphocytes on microscope slides were used as a test system. In these experiments, three fluorescent antibodies were used to identify lymphocyte sub-populations, and a DNA content probe was used to identify all nucleated cells. The cell subset percentages determined by image cytometry were comparable to percentages obtained when cells from the same preparation were analyzed by flow cytometry. Multicolor fluorescence imaging cytometry can potentially be extended to the analysis of cells in smears, fine needle biopsies, imprints, and tissue sections.

引用

页码：579 / 596

页数：18

共 20 条

[1] FLUORESCENT LOW-DENSITY LIPOPROTEIN FOR OBSERVATION OF DYNAMICS OF INDIVIDUAL RECEPTOR COMPLEXES ON CULTURED HUMAN-FIBROBLASTS [J].

BARAK, LS ;

WEBB, WW .

JOURNAL OF CELL BIOLOGY, 1981, 90 (03) :595-604

[2]

BRELJE TC, UNPUB J HISTOCHEM CY

[3] PROPOSED STANDARD FOR IMAGE CYTOMETRY DATA FILES [J].

DEAN, P ;

MASCIO, L ;

OW, D ;

SUDAR, D ;

MULLIKIN, J .

CYTOMETRY, 1990, 11 (05) :561-569

[4] 5-PARAMETER FLUORESCENCE IMAGING - WOUND-HEALING OF LIVING SWISS 3T3 CELLS [J].

DEBIASIO, R ;

BRIGHT, GR ;

ERNST, LA ;

WAGGONER, AS ;

TAYLOR, DL .

JOURNAL OF CELL BIOLOGY, 1987, 105 (04) :1613-1622

[5] CYANINE DYE LABELING REAGENTS FOR SULFHYDRYL-GROUPS [J].

ERNST, LA ;

GUPTA, RK ;

MUJUMDAR, RB ;

WAGGONER, AS .

CYTOMETRY, 1989, 10 (01) :3-10

[6] MULTIPLE SPECTRAL PARAMETER IMAGING IN QUANTITATIVE FLUORESCENCE MICROSCOPY .1. QUANTITATION OF BEAD STANDARDS [J].

GALBRAITH, W ;

RYAN, KW ;

GLIKSMAN, N ;

TAYLOR, DL ;

WAGGONER, AS .

COMPUTERIZED MEDICAL IMAGING AND GRAPHICS, 1989, 13 (01) :47-60

[7]

GALBRAITH W, 1989, P SOC PHOTO-OPT INS, V1063, P74

[8]

HART L, 1989, ANAL QUANT CYT HISTO, P127

[9] IMAGING-SYSTEM FOR MORPHOMETRIC ASSESSMENT OF ABSORPTION OR FLUORESCENCE IN STAINED CELLS [J].

JAGGI, B ;

POON, SSS ;

MACAULAY, C ;

PALCIC, B .

CYTOMETRY, 1988, 9 (06) :566-572

[10] LUMINESCENCE DIGITAL IMAGING MICROSCOPY [J].

JOVIN, TM ;

ARNDTJOVIN, DJ .

ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY, 1989, 18 :271-308

← 1 2 →