MAPPING THE 3-DIMENSIONAL LOCATIONS OF RIBOSOMAL-RNA AND PROTEINS

被引:20
作者
SCHEINMAN, A
ATHA, T
AGUINALDO, AM
KAHAN, L
SHANKWEILER, G
LAKE, JA
机构
[1] UNIV CALIF LOS ANGELES,INST MOLEC BIOL,LOS ANGELES,CA 90024
[2] UNIV WISCONSIN,DEPT PHYSIOL CHEM,MADISON,WI 53711
[3] UNIV CALIF LOS ANGELES,DEPT BIOL,LOS ANGELES,CA 90024
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
RIBOSOMAL RNA; RIBOSOMAL PROTEINS; DNA HYBRIDIZATION ELECTRON MICROSCOPY;
D O I
10.1016/0300-9084(92)90108-Q
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Seven regions of 16S rRNA have been located on the surface of the 30S ribosomal subunit by DNA hybridization electron microscopy in our laboratory. In addition, we have recently mapped the three-dimensional locations of an additional seven small ribosomal proteins by immunoelectron microscopy. The information from the direct mapping of the sites on rRNA has been incorporated into a model for the tertiary structure of 16S rRNA, accounting for approximately 40% of the total 16S rRNA. A novel structure, the platform ring, is proposed for a region of rRNA within the central domain. This structure rings the edges of the platform and includes regions 655-751 and 769-810. Another region, the recognition complex, consists of nucleotides 500-545, and occupies a region on the exterior surface of the subunit, near the EF-Tu binding site. In addition, 19 of the 21 small subunit ribosomal proteins have been mapped by immunoelectron microscopy in our laboratory. In order to evaluate the reliability of our model for the three-dimensional distribution of 16S rRNA, we have predicted which sites of rRNA are adjacent to ribosomal proteins and compared these predictions with r-protein protection studies of others. Good correlation between the model, the locations of rRNA sites, the locations of ribosomal proteins, and regions of rRNA protected by ribosomal proteins, provides independent support for this model.
引用
收藏
页码:307 / 317
页数:11
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