DIFFERENTIAL PROLIFERATIVE AND INTERLEUKIN-10 RESPONSES TO FRACTIONATED FILARIAL ANTIGENS - PREFERENTIAL RECOGNITION BY PATIENTS WITH CHRONIC LYMPHATIC DYSFUNCTION
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作者:
DIMOCK, KA
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机构:Division of Parasitic Diseases, National Centers for Infectious Diseases, Centers for disease control and prevention, US department of health and human services, Atlanta, GA
DIMOCK, KA
ADDISS, DG
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机构:Division of Parasitic Diseases, National Centers for Infectious Diseases, Centers for disease control and prevention, US department of health and human services, Atlanta, GA
ADDISS, DG
EBERHARD, ML
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机构:Division of Parasitic Diseases, National Centers for Infectious Diseases, Centers for disease control and prevention, US department of health and human services, Atlanta, GA
EBERHARD, ML
LAMMIE, PJ
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机构:Division of Parasitic Diseases, National Centers for Infectious Diseases, Centers for disease control and prevention, US department of health and human services, Atlanta, GA
LAMMIE, PJ
机构:
[1] Division of Parasitic Diseases, National Centers for Infectious Diseases, Centers for disease control and prevention, US department of health and human services, Atlanta, GA
To characterize filarial antigens that may be associated with the development of chronic lymphatic dysfunction in persons with lymphatic filariasis, T cell responsiveness to Brugia pahangi adult worm extracts and SDS-PAGE antigen fractions were examined among Haitians from an area in which Wuchereria bancrofti is endemic. Greater T cell proliferation and interleukin-10 (IL-10) production were observed in amicrofilaremic patients with hydrocele or elephantiasis than in amicrofilaremic or microfilaremic asymptomatic persons. Antigen fractions that stimulated the highest proliferative responses (in the 25-49 kDa range) and IL-10 production were not identical. Further separation of an immunodominant 30- to 38-kDa fraction by ion exchange high-pressure liquid chromatography identified several subfractions, including a 32-kDa protein band, that elicited T cell responses from patients with elephantiasis or hydrocele. By immunoblot, these patients also had markedly greater humoral reactivity to parasite antigens of similar to 52, 43, 32, and 30 kDa.