INDUCTION OF APOPTOSIS BY ACYCLIC RETINOID IN THE HUMAN HEPATOMA-DERIVED CELL-LINE, HUH-7

被引：85

作者：

NAKAMURA, N ^{[1
]}

SHIDOJI, Y ^{[1
]}

YAMADA, Y ^{[1
]}

HATAKEYAMA, H ^{[1
]}

MORIWAKI, H ^{[1
]}

MUTO, Y ^{[1
]}

机构：

[1] GIFU UNIV,SCH MED,DEPT INTERNAL MED 1,GIFU 500,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 207卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1199

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HuH-7 cells, a human hepatoma-derived cell line, underwent apoptosis in response to all-trans 3, 7, 11, 15-tetramethyl-2, 4, 6, 10, 14-hexadecapentaenoic acid, or acyclic retinoid. The retinoid-induced apoptosis was verified by a characteristic step-wise fragmentation of genomic DNA and chromatin condensation. The induction of apoptosis was detected as early as 8 hrs after the addition of the retinoid and was concentration dependent(0.1 similar to 10 mu M). Neither the natural retinoid all-trans retinoic acid nor 9-cis retinoic acid induced apoptosis. These data strongly indicate that the antitumor activity of the acyclic retinoid may be partly explained by the induction of apoptosis in tumor cells. (C) 1995 Academic Press, Inc.

引用

页码：382 / 388

页数：7

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