VASOACTIVE INTESTINAL POLYPEPTIDE STIMULATES CYCLIC-AMP PRODUCTION IN MOUSE N1E-115 NEUROBLASTOMA-CELLS - MODULATION BY A PROTEIN-KINASE-C ACTIVATOR AND IONOMYCIN

被引：6

作者：

INUKAI, T

CHIK, CL

HO, AK

机构：

[1] UNIV ALBERTA,FAC MED,DEPT PHYSIOL,EDMONTON T6G 2H7,AB,CANADA

[2] UNIV ALBERTA,FAC MED,DEPT MED,EDMONTON T6G 2H7,AB,CANADA

来源：

PEPTIDES | 1994年 / 15卷 / 08期

基金：

英国医学研究理事会;

关键词：

CAMP; NEUROBLASTOMA CELLS; PROTEIN KINASE C; IONOMYCIN; VIP;

D O I：

10.1016/0196-9781(94)90109-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we investigated the vasoactive intestinal polypeptide (VIP)-stimulated cAMP production and its interaction with protein kinase C activation and elevation of intracellular Ca2+ in N1E-115 neuroblastoma cells. VIP treatment caused a 55-fold increase in cAMP accumulation. Addition of 4 beta-phorbol 12-myristate 13-acetate reduced VIP- but not forskolin-stimulated cAMP response. In comparison, ionomycin potentiated both VIF- and forskolin-induced cAMP accumulation. Our results indicate that VIP stimulates cAMP accumulation in N1E-115 cells, and that although activation of protein kinase C inhibits the VIP-stimulated cAMP response, elevation of intracellular Ca2+ potentiates this signaling pathway.

引用

页码：1361 / 1365

页数：5

共 22 条

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