5'-TERMINUS OF BACTERIOPHAGE R17 RNA - PPPGP

Alkaline hydrolysis of radioactive R17 RNA made in vivo yields pppGp from the 5′-terminus of the RNA. 0.75 mole pppGp/mole of RNA has been recovered by chromatography of the alkaline hydrolysate on DEAE-Sephadex columns using buffers containing 7 m-urea and NaCl gradient elution. Various degradative experiments have been carried out which prove the structure of pppGp. This nucleoside 5′-triphosphate group can be used as a natural label of the 5′-terminus for studies of the 5′-terminal nucleotide sequence of R17 RNA. © 1968.