SUBTRACTION HYBRIDIZATION CDNA LIBRARIES FROM COLON-CARCINOMA AND HEPATIC CANCER

被引：76

作者：

SCHWEINFEST, CW

HENDERSON, KW

GU, JR

KOTTARIDIS, SD

BESBEAS, S

PANOTOPOULOU, E

PAPAS, TS

机构：

[1] NCI,FREDERICK CANC RES FACIL,PROGRAM RESOURCES INC,FREDERICK,MD 21701

[2] SHANGHAI CANC INST,DEPT BIOCHEM & MOLEC BIOL,SHANGHAI,PEOPLES R CHINA

[3] HELLEN ANTICANC INST,DEPT VIROL,GR-11532 ATHENS,GREECE

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1990年 / 7卷 / 03期

关键词：

D O I：

10.1016/0735-0651(90)90042-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA clones of differentially expressed mRNAs in a colon carcinoma and a hepatocellular carcinoma have been isolated by subtractive cDNA cloning. The subtracted material is at least 90 × enriched for differentially expressed sequences and can be used for construction of subtractive cDNA libraries and polymerase chain reaction (PCR) amplification to generate differential probes. Commercially available λZAP II is used for construction of primary libraries since single-stranded phage bearing the cloned cDNA can be excised in vivo and because λ libraries are convenient for subsequent screening and manipulations. Rare mRNAs (less than 0.01% abundance), which are differentially expressed, can be isolated utilizing this procedure. © 1990.

引用

页码：64 / 70

页数：7

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