A ONE-STEP SANDWICH ENZYME-IMMUNOASSAY FOR GAMMA-CARBOXYLATED OSTEOCALCIN USING MONOCLONAL-ANTIBODIES

被引：44

作者：

KOYAMA, N ^{[1
]}

OHARA, K ^{[1
]}

YOKOTA, H ^{[1
]}

KUROME, T ^{[1
]}

KATAYAMA, M ^{[1
]}

HINO, F ^{[1
]}

KATO, I ^{[1
]}

AKAI, T ^{[1
]}

机构：

[1] YODA HOSP,SENNAN,OSAKA,JAPAN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 139卷 / 01期

关键词：

MONOCLONAL ANTIBODY; SANDWICH ENZYME IMMUNOASSAY; OSTEOCALCIN; GAMMA-CARBOXYLATION;

D O I：

10.1016/0022-1759(91)90346-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive, simple and reliable one-step sandwich enzyme immunoassay (EIA) for the gamma-carboxylated form of osteocalcin (Gla-OC) has been developed using a monoclonal antibody. The minimum amount of Gla-OC detected by this EIA was approximately 0.2 ng/ml when a 10-mu-l aliquot of the sample was used. The serum Gla-OC level in 30 healthy subjects was 3.6 +/- 2.19 ng/ml (mean +/- SD). A significant increase was seen in patients with chronic renal failure (20.3 +/- 4.60 ng/ml), atherosclerosis (8.3 +/- 4.94 ng/ml) and osteoporosis (10.1 +/- 4.60 ng/ml). The correlation between the values obtained by the sandwich EIA and competitive RIA methods was given by the linear regression equation, y = 2.896 + 0.759x, for which the correlation coefficient (r) was 0.815 (n = 58). This newly developed Gla-OC specific EIA may be useful for the diagnosis of metabolic bone disease and ectopic calcification.

引用

页码：17 / 23

页数：7

共 22 条

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