CHARACTERIZATION OF APO(A) POLYMORPHISM BY A MODIFIED IMMUNOBLOTTING TECHNIQUE IN AN ITALIAN POPULATION-SAMPLE

被引：42

作者：

GEROLDI, D ^{[1
]}

BELLOTTI, V ^{[1
]}

BUSCAGLIA, P ^{[1
]}

BONETTI, G ^{[1
]}

GAZZARUSO, C ^{[1
]}

CAPRIOLI, A ^{[1
]}

FRATINO, P ^{[1
]}

机构：

[1] UNIV PAVIA,DEPT BIOCHEM,PAVIA,ITALY

来源：

CLINICA CHIMICA ACTA | 1993年 / 221卷 / 1-2期

关键词：

LIPOPROTEIN(A); IMMUNOBLOTTING; APO(A) ISOFORMS;

D O I：

10.1016/0009-8981(93)90030-8

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Apo(a), the specific lipoprotein(a) (Lp(a)) apolipoprotein, is characterized by different isoforms (from 6 to 11 on SDS-PAGE) encoded by a system of autosomal codominant alleles. Electrophoresis on agarose gel displays a better resolving power than SDS-PAGE (a larger number of apo(a) isoforms is detected). The aim of this work was to set up a simple technique that uses a capillary blotting apparatus and a polyvinylidene difluoride membrane for protein transfer. We tested an Italian population sample of 202 healthy subjects (123 men and 79 women) and we detected 22 apo(a) isoforms varying from 280 to 775 kDa. In our sample, 135 subjects (66.5%) had a single-band phenotype, 64 (31.7%) had a double-band phenotype and 3 subjects (1.5%) had no detectable bands ('null' phenotype). This simple and reproducible technique could be applied in the genetic screening of apo(a) polymorphisms and for clinical investigations of the risk of developing cardiovascular diseases.

引用

页码：159 / 169

页数：11

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