CONFIRMATION OF DANOFLOXACIN RESIDUES IN CHICKEN AND CATTLE LIVER BY MICROBORE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY-IONIZATION TANDEM MASS-SPECTROMETRY

被引：31

作者：

SCHNEIDER, RP ^{[1
]}

ERICSON, JF ^{[1
]}

LYNCH, MJ ^{[1
]}

FOUDA, HG ^{[1
]}

机构：

[1] PFIZER INC,DIV LABS & RES,DEPT DRUG METAB,EASTERN POINT RD,GROTON,CT 06340

来源：

BIOLOGICAL MASS SPECTROMETRY | 1993年 / 22卷 / 10期

关键词：

D O I：

10.1002/bms.1200221007

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A specific assay is described for the confirmatory identification of danofloxacin residues in edible tissues of cattle and chicken. The assay utilizes on-line microbore high-performance liquid chromatography and pneumatically assisted electrospray tandem mass spectrometry (MS/MS). Collision-induced dissociation of the danofloxacin protonated molecule results in two significant daughter ions. Monitoring both ions provides the specificity required for this confirmatory assay. Optimum electrospray and MS/MS operating conditions permitted the specific monitoring of danofloxacin and the confirmation of its residues in chicken and cattle liver extracts down to 50 ppb. The analysis of control liver or the commercially available antibacterial quinolones enrofloxacin and its metabolite ciprofloxacin gave no response under the assay conditions. The ratios of the two daughter ions were similar for danofloxacin standard solutions, fortified tissues and incurred tissues.

引用

页码：595 / 599

页数：5

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