SYNTHESIS OF HOLO PARACOCCUS-DENITRIFICANS CYTOCHROME C(550) REQUIRES TARGETING TO THE PERIPLASM WHEREAS THAT OF HOLO HYDROGENOBACTER-THERMOPHILUS CYTOCHROME C(552) DOES NOT - IMPLICATIONS FOR C-TYPE CYTOCHROME BIOGENESIS

Expression from a plasmid of the complete gene, including the codons for the N-terminal periplasmic targeting signal, for cytochrome c(550) of Paracoccus dentrificans led to the formation of the hole protein in the periplasms of both P. dentrificans and Escherichia coli. Expression of the gene from which the region coding for the targeting signal had been specifically deleted resulted in formation of ape-protein in the cytoplasms of both organisms. These findings are consistent with haem attachment occurring in the periplasm. In contrast, the formation of holo cytochrome c(552) from Hydrogenobacter thermophilus following expression of the gene lacking the periplasmic targeting sequence in either P. denitrificans or E. coli is attributed to spontaneous cytoplasmic attachment of haem to the thermostable protein.