DISTINCT CELLULAR-LOCALIZATION OF MESSENGER-RNAS FOR 3 SUBTYPES OF PROSTAGLANDIN-E RECEPTOR IN KIDNEY

Distribution of the mRNAs for three subtypes ofprostaglandin E (PGE) receptors in the mouse kidney was investigated by in situ hybridization. The mRNA for EP(1) subtype, which is coupled to Ca2+ mobilization, was specifrcally localized to the collecting ducts from the cortex to the papilla. The mRNA for EP(2) subtype, which is linked to stimulation of adenylate cyclase, was localized to the glomeruli. The mRNA for EP(3) subtype, which is coupled to inhibition of adenylate cyclase, was located densely in the tubules in the outer medulla and in the distal tubules in the cortex. These results exhibit distinct cellular localization of three subtypes of PGE receptor in the kidney and suggest that PGE(2) exerts multiple functions via these subtypes expressed in different segments of the nephron.