SPECIFIC OLIGONUCLEOTIDE PRIMERS FOR DETECTION OF LECITHINASE-POSITIVE BACILLUS SPP BY PCR

被引：53

作者：

SCHRAFT, H ^{[1
]}

GRIFFITHS, MW ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT FOOD SCI,GUELPH,ON N1G 2W1,CANADA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 01期

关键词：

D O I：

10.1128/AEM.61.1.98-102.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An assay based on the PCR has been developed to facilitate detection and identification of Bacillus cereus in foods. Three primers for the PCR have been designed within the sequence for cereolysin AB, a cytolytic determinant that encodes lecithin-hydrolyzing and hemolytic activities of B. cererus. With the PCR and hybridization, the specificity of the primers was tested with 39 isolates of the B. cereus group, with 17 other Bacillus spp., and with 21 non-Bacillus strains. Results demonstrate a high specificity of the three oligonucleotides for isolates of the B. cereus group. With a combined PCR-hybridization assay, the detection limit for B. cereus in artificially contaminated milk nas 1 CFU/ml of milk.

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页码：98 / 102

页数：5

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