HETEROLOGOUS EXPRESSION OF AN ACTIVE-RAT REGULATORY PROTEIN OF GLUCOKINASE

被引：6

作者：

DETHEUX, M

VANSCHAFTINGEN, E

机构：

[1] UNIV CATHOLIQUE LOUVAIN,CHIM PHYSIOL LAB,B-1200 BRUSSELS,BELGIUM

[2] INT INST CELLULAR & MOLEC PATHOL,B-1200 BRUSSELS,BELGIUM

来源：

FEBS LETTERS | 1994年 / 355卷 / 01期

关键词：

GLUCOKINASE; GLYCOLYSIS; FRUCTOSE; 1-PHOSPHATE; 6-PHOSPHATE;

D O I：

10.1016/0014-5793(94)01160-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA presumed to encode the rat liver regulatory protein of glucokinase has been expressed in Escherichia coli and a partially soluble protein has been obtained. This recombinant protein was partially purified and found to have the same apparent molecular mass as the regulatory protein purified from rat liver. Like the latter, it inhibited rat liver glucokinase competitively with respect to glucose and its effect was sensitive to fructose 6-phosphate and fructose 1-phosphate.

引用

收藏

页码：27 / 29

页数：3

相关论文

共 13 条

[1]

DACUNHA MV, 1994, EUR J BIOCHEM, V225, P43

[2]

DETHEUX M, 1994, FEBS LETT, V339, P312

[3] EFFECTORS OF THE REGULATORY PROTEIN ACTING ON LIVER GLUCOKINASE - A KINETIC INVESTIGATION [J].

DETHEUX, M ;

VANDERCAMMEN, A ;

VANSCHAFTINGEN, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 200 (02) :553-561

[4] CLONING AND SEQUENCING OF RAT-LIVER CDNAS ENCODING THE REGULATORY PROTEIN OF GLUCOKINASE [J].

DETHEUX, M ;

VANDEKERCKHOVE, J ;

VANSCHAFTINGEN, E .

FEBS LETTERS, 1993, 321 (2-3) :111-115

[5] HOMOLOGOUS PLANT AND BACTERIAL PROTEINS CHAPERONE OLIGOMERIC PROTEIN ASSEMBLY [J].

HEMMINGSEN, SM ;

WOOLFORD, C ;

VANDERVIES, SM ;

TILLY, K ;

DENNIS, DT ;

GEORGOPOULOS, CP ;

HENDRIX, RW ;

ELLIS, RJ .

NATURE, 1988, 333 (6171) :330-334

[6] CONSTRUCTION OF T-VECTORS, A RAPID AND GENERAL SYSTEM FOR DIRECT CLONING OF UNMODIFIED PCR PRODUCTS [J].

MARCHUK, D ;

DRUMM, M ;

SAULINO, A ;

COLLINS, FS .

NUCLEIC ACIDS RESEARCH, 1991, 19 (05) :1154-1154

[7] USE OF BACTERIOPHAGE-T7 RNA-POLYMERASE TO DIRECT SELECTIVE HIGH-LEVEL EXPRESSION OF CLONED GENES [J].

STUDIER, FW ;

MOFFATT, BA .

JOURNAL OF MOLECULAR BIOLOGY, 1986, 189 (01) :113-130

[8] ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].

TOWBIN, H ;

STAEHELIN, T ;

GORDON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354

[9] SPECIES AND TISSUE DISTRIBUTION OF THE REGULATORY PROTEIN OF GLUCOKINASE [J].

VANDERCAMMEN, A ;

VANSCHAFTINGEN, E .

BIOCHEMICAL JOURNAL, 1993, 294 :551-556

[10] THE MECHANISM BY WHICH RAT-LIVER GLUCOKINASE IS INHIBITED BY THE REGULATORY PROTEIN [J].

VANDERCAMMEN, A ;

VANSCHAFTINGEN, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 191 (02) :483-489