CHARACTERIZATION OF RAT SPLEEN PROSTAGLANDIN-H D-ISOMERASE AS A SIGMACLASS GSH TRANSFERASE

被引：138

作者：

MEYER, DJ

THOMAS, M

机构：

[1] Dept Biochem and Molecular Biology, University College, London, London W1P 6DB, Cleveland Street

来源：

BIOCHEMICAL JOURNAL | 1995年 / 311卷

关键词：

D O I：

10.1042/bj3110739

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The prostaglandin H D-isomerase of rat immune accessory cells has been purified from spleen by a simple procedure, and its high specificity and activity [Urade, Fujimoto, Ujihara and Hayaishi (1987) J. Biol. Chem. 262, 3820-3825] have been confirmed in an assay coupled to prostaglandin H synthase. The enzyme also decreases the formation of 12[S]-hydroxy-5,8,10-heptadecatrienoic ac:id formed by the synthase in the presence of GSH and increases the overall rate of arachidonate oxidation. A partial amino acid sequence shows a strong relationship to GSH transferases of parasitic helminths and molluscs, indicating that it is the first example of a vertebrate sigma-class GSH transferase, and suggesting that certain helminth GSH transferases may be involved in prostaglandin synthesis.

引用

页码：739 / 742

页数：4

共 28 条

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