ACCUMULATION OF EARLY MITOTIC CELLS IN ETHIDIUM BROMIDE-TREATED HUMAN LYMPHOCYTE-CULTURES

被引：97

作者：

IKEUCHI, T

SASAKI, M

机构：

[1] Chromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 1979年 / 55卷 / 01期

关键词：

D O I：

10.2183/pjab.55.15

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

When human lymphocyte cultures are exposed to EB (10 pg/ml) for 2 hr prior to fixation, there is a marked increase in frequency of early mitotic cells, possibly due to the prolongation of the prophase-metaphase transition. This procedure may serve as a simple method for obtaining a large number of cells with extremely elongated chromosomes, which would permit a great precision of human chromosome analyses. © 1979, The Japan Academy. All rights reserved.

引用

页码：15 / 18

页数：4

共 12 条

[1] MAPPING G-BANDS ON HUMAN PROPHASE CHROMOSOMES [J].