ISOLATION OF PURE LAC OPERON DNA

被引：176

作者：

SHAPIRO, J

MACHATTI.L

ERON, L

IHLER, G

IPPEN, K

BECKWITH, J

机构：

[1] Department of Bacteriology and Immunology, Harvard Medical School, Boston

[2] Department of Cell Biology, University of Toronto

[3] Department of Biochemistry, University of Pittsburgh, Pittsburgh, PA

[4] MRC Microbial Genetics Research Unit, Department of Molecular Biology, University of Edinburgh, Edinburgh

来源：

NATURE | 1969年 / 224卷 / 5221期

关键词：

D O I：

10.1038/224768a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Two specialized transducing phages, which carry the lac operon of Escherichia coli inserted into their DNA in opposite orientations, are used as a source of complementary sequences from which to prepare pure lac operon duplex. The availability of a single pure promoter will facilitate many new experiments on genetic transcription. © 1969 Nature Publishing Group.

引用

页码：768 / +

页数：1

共 31 条

[1] TRANSPOSITION OF LAC REGION OF ESCHERICHIA COLI .I. INVERSION OF LAC OPERON AND TRANSDUCTION OF LAC BY PHI80 [J].